Malate Dehydrogenase, IFCC Quality
from pig heart, solution
For further processing only.
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Will be supplied as "MDH, IFCC-quality, Pig Heart (Mitochon.)". Unit of measure is "MU".
Malate Dehydrogenase, IFCC Quality material number and pack size: Material Number Pack Size 10417726103 custom fill - Dehydrogenase that catalyzes the interconversion of malate to oxaloacetate.
- Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.
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Nomenclature: L-malate:NAD+ oxidoreductase
Molecular weight: 70 kD
Isoelectric point: 6.1-6.4
Michaelis constants (Phosphate buffer, 95 mmol/L, pH 8.3, +25°C):
L-malate: 4.0 x 10-4 mol/L
L-tartrate: 9.0 x 10-3 mol/L
meso-tartrate: 1.2 x 10-3 mol/L
oxaloacetate: 3.3 x 10-5 mol/L
Inhibitors: Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Activators: Phosphate, arsenate, Zn2+
pH optimum: 7.5 (see figure for product Cat. No. 10 267 155 103)
Temperature dependence: See figure for product Cat. No. 10 267 155 103
pH stability: 7.0-9.0 (see figure for product Cat. No. 10 267 155 103)
Thermal stability: Up to +40°C (see figure for product Cat. No. 10 267 155 103)
Specificity: L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP. Appearance: Clear, colorless solution in glycerol (50% (v/v))
pH value: 6.5-7.5
Specific activity (+30°C, oxaloacetate): ≥900 U/mg protein
Protein (Biuret): ≥10 mg/mL
Contaminants (expressed as percentage of Malate Dehydrogenase activity):
Aspartate aminotransferase (AST/GOT): ≤0.005
Alanine aminotransferase (ALT/GPT): ≤0.005
Glutamate dehydrogenase: ≤0.003
Reagent blank for determination of aspartate aminotransferase (AST/GOT): ≤0.009 (A334/10 minutes)
SVD free: Corresponds to specification
pH 5.5 treatment (for at minimum 30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months. -
