Malate Dehydrogenase, IFCC Quality
from pig heart, lyophilizate
For further processing only.
expand | collapse
Malate Dehydrogenase, IFCC Quality material number and pack size: Material Number Pack Size 10267155103 custom fill
- Dehydrogenase that catalyzes the interconversion of malate to oxaloacetate.
- Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.
Nomenclature: L-malate:NAD+ oxidoreductase
Molecular weight: 70 kD
Isoelectric point: 6.1-6.4
Michaelis constants (Phosphate buffer, 95 mmol/L, pH 8.3, +25°C):
L-malate: 4.0 x 10-4 mol/L
L-tartrate: 9.0 x 10-3 mol/L
meso-tartrate: 1.2 x 10-3 mol/L
oxaloacetate: 3.3 x 10-5 mol/L
Inhibitors: Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Activators: Phosphate, arsenate, Zn2+
pH optimum: 7.5 (see figure)
Temperature dependence: See figure
pH stability: 7.0-9.0 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP. Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=10 mg/mL)
Activity (+30°C, oxaloacetate; according to the IFCC recommendations): ≥70 U/mg lyophilizate
Contaminants (expressed as percentage of Malat Dehydrogenase activity; assayed according to the IFCC recommendations):
Aspartate aminotransferase (AST/GOT): ≤0.001
Alanine aminotransferase (ALT/GPT): ≤0.001
Glutamate dehydrogenase: ≤0.005
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months. Store dry.