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Malate Dehydrogenase, IFCC Quality

from pig heart, lyophilizate

For further processing only.

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Order information
Malate Dehydrogenase, IFCC Quality material number and pack size:
Material Number Pack Size
10267155103 custom fill
Will be supplied as "MDH IFCC-quality, Pig Heart (Mitochon.)". Unit of measure is "MU".
Dehydrogenase that catalyzes the interconversion of malate to oxaloacetate.
Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.
Nomenclature: L-malate:NAD+ oxidoreductase
Molecular weight: 70 kD
Isoelectric point: 6.1-6.4
Michaelis constants (Phosphate buffer, 95 mmol/L, pH 8.3, +25°C):
L-malate: 4.0 x 10-4 mol/L
L-tartrate: 9.0 x 10-3 mol/L
meso-tartrate: 1.2 x 10-3 mol/L
oxaloacetate: 3.3 x 10-5 mol/L
Inhibitors: Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Activators: Phosphate, arsenate, Zn2+
pH optimum: 7.5 (see figure)
Temperature dependence: See figure
pH stability: 7.0-9.0 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP.
Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=10 mg/mL)
Activity (+30°C, oxaloacetate; according to the IFCC recommendations): ≥70 U/mg lyophilizate
Contaminants (expressed as percentage of Malat Dehydrogenase activity; assayed according to the IFCC recommendations):
Aspartate aminotransferase (AST/GOT): ≤0.001
Alanine aminotransferase (ALT/GPT): ≤0.001
Glutamate dehydrogenase: ≤0.005
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months. Store dry.