Ascorbate Oxidase
from Cucurbita species, poor of AST/ALT, lyophilizate
For further processing only.
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Will be supplied as "Ascorbate Oxidase GOT-deficient". Unit of measure is "MU".
Ascorbate Oxidase material number and pack size: Material Number Pack Size 11136364103 custom fill - Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate.
- Use Ascorbate Oxidase, poor of AST/ALT, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate, creatinine or transaminases.
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Nomenclature: L-ascorbate:oxygen oxidoreductase
Molecular weight: Approximately 140 kD
Isoelectric point: 5.0-6.0
Michaelis constant (Phosphate buffer, pH 5.6, +25°C):
L-ascorbate: 3 x 10-4 mol/L
Inhibitors: 4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
pH optimum: 5.6-7.0 (see figure for product Cat. No. 10 199 605 103)
Temperature dependence: See figure for product Cat. No. 10 199 605 103
pH stability: 6.5-9.0 (see figure for product Cat. No. 10 199 605 103)
Thermal stability: Up to +70°C (see figure for product Cat. No. 10 199 605 103)
Stability of the lyophilizate: Stable at +35°C for at least 3 weeks (see figure).
Stability in solution: See figure for product Cat. No. 10 199 605 103
Specificity: Several analogs of ascorbate react.
Remark: A decrease in activity of approximately 10% may occur. Appearance: Turquoise lyophilizate
Solubility: Clear, slightly turquoise solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, L-ascorbate): ≥170 U/mg lyophilizate
Specific activity (+25°C): ≥1,700 U/mg protein
Protein (Biuret): 8-14 mg/100mg lyophilizate
Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):
Catalase: ≤0.2
Aspartate aminotransferase (AST/GOT): ≤0.0003
Alanine aminotransferase (ALT/GPT): ≤0.0005
Contaminating oxidases (FOX): ≤0.0002
Stability: At -15 to -25°C within specification range for 12 months. Keep tightly sealed.