{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "beta-Galactosidase recombinant EIA Grade", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "β-Galactosidase, recombinant, EIA Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.7.3.1.2.1", "ProductNameAddition": "from E. coli overproducer, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Immunology Raw Materials", "NodeID": "02-0035" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Glycosidases", "NodeID": "70-024-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10570079103", "MaterialDescription": "b-Galactosidase, Escherichia coli", "RegisteredProductName": "beta-Galactosidase, recombinant, EIA Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"β-Galactosidase, E. coli\". Unit of measure is \"g active ingredient\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"β-Galactosidase, E. coli\". Unit of measure is \"g active ingredient\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "β-Galactosidase hydrolyzes ß-D-galactosides. Although the enzyme activity with 2-nitrophenyl-β-D-galactoside as substrate is higher than with the 4-isomer, the enzyme reaction with the 4-compound is more sensitive due to a higher absorption coefficient for 4-nitrophenol, ε405: 18.5 [mmol-1 x l x cm-1].", "Name": "Background Information" }, { "Language": "en", "Value": "Marker enzyme for the manufacturing of antibody- and antigen-enzyme conjugates incorporated in immunoassays for colorimetric and fluorimetric detection.", "Name": "Applications" }, { "Language": "en", "Value": "Nomenclature: β-D-galactohydrolase
CAS Number: 9031-11-2​​​​​​​
Molecular weight (by sequence): 465 kD
Structure: 4 identical subunits, β-galactosidase contains no carbohydrates
Isoelectric point: 4.61
Michaelis constants:
Tris buffer, pH 7.6, +20°C / relation rate:
2-nitrophenyl-β-galactoside: 9.50 x 10-4 mol/L / 1.00
phenyl-β-D-galactoside: 3.23 x 10-3 mol/L / 0.05
lactose: 3.85 x 10-2 mol/L / 0.06
4-nitrophenyl-β-galactoside: 4.45 x 10-4 mol/L / ~0.50
Activators: Mg2+ and Na+ (or other monovalent cations) are essential for activity.
pH optimum: 8.0
pH stability: 6.0
Thermal stability: Up to +37°C
Thiol groups: 64 SH groups, approximately 16 of these are accessible for SH-reactive reagents. 4 of these (Cys 76) take part in conjugation.", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White lyophilizate, stabilized with phosphate buffer and sucrose
Solubility: Clear, colorless solution in water (c=20 mg/mL)
Protein (Biuret): Approximatly 0.25 mg/mg lyophilizate
Specific activity (+37°C, 2-nitrophenyl-β-D-galactopyranoside): ≥700 U/mg protein
SH-groups, free (after dialysis): ≥12 mol/mol enzyme (corresponds to 465,000 g)
Aggregated β-galactosidase (HPLC): ≤3 area% (dimer-part with a molar mass of 0.93 x 106 D)
Stability: At -15 to -25°C within specification range for 24 months. Store under nitrogen.", "Name": "Specification" } ] } } ] }

β-Galactosidase, recombinant, EIA Grade

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