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""
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"MaterialDescription": "b-Galactosidase, Escherichia coli",
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"Name": "CB - Order Information",
"Value": "Will be supplied as \"β-Galactosidase,
E. coli\". Unit of measure is \"g active ingredient\".",
"Language": "en",
"Country": "XG",
"Code": "CB - Order Information"
},
{
"Name": "Background Information",
"Value": "β-Galactosidase hydrolyzes ß-D-galactosides. Although the enzyme activity with 2-nitrophenyl-β-D-galactoside as substrate is higher than with the 4-isomer, the enzyme reaction with the 4-compound is more sensitive due to a higher absorption coefficient for 4-nitrophenol, ε
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Nomenclature: β-D-galactohydrolase
CAS Number: 9031-11-2
Molecular weight (by sequence): 465 kD
Structure: 4 identical subunits, β-galactosidase contains no carbohydrates
Isoelectric point: 4.61
Michaelis constants:
Tris buffer, pH 7.6, +20°C / relation rate:
2-nitrophenyl-β-galactoside: 9.50 x 10
-4 mol/L / 1.00
phenyl-β-D-galactoside: 3.23 x 10
-3 mol/L / 0.05
lactose: 3.85 x 10
-2 mol/L / 0.06
4-nitrophenyl-β-galactoside: 4.45 x 10
-4 mol/L / ~0.50
Activators: Mg
2+ and Na
+ (or other monovalent cations) are essential for activity.
pH optimum: 8.0
pH stability: 6.0
Thermal stability: Up to +37°C
Thiol groups: 64 SH groups, approximately 16 of these are accessible for SH-reactive reagents. 4 of these (Cys 76) take part in conjugation.",
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"Country": "XG",
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Appearance: White lyophilizate, stabilized with phosphate buffer and sucrose
Solubility: Clear, colorless solution in water (c=20 mg/mL)
Protein (Biuret): Approximatly 0.25 mg/mg lyophilizate
Specific activity (+37°C, 2-nitrophenyl-β-D-galactopyranoside): ≥700 U/mg protein
SH-groups, free (after dialysis): ≥12 mol/mol enzyme (corresponds to 465,000 g)
Aggregated β-galactosidase (HPLC): ≤3 area% (dimer-part with a molar mass of 0.93 x 10
6 D)
Stability: At -15 to -25°C within specification range for 24 months. Store under nitrogen.",
"Language": "en",
"Country": "XG",
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