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FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

For further processing only.

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Order information
FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl material number and pack size:
Material Number Pack Size
04659163103 5 kU
Will be supplied as "FastStart Taq DNA Pol. Ind. GMP Grd, 5KU". Unit of measure is "piece".
The enzyme is supplied without reaction buffer.
Hot start Taq DNA Polymerase for highly specific and sensitive amplification using PCR.
  • Achieve high specificity, sensitivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
  • Obtain reliable results.
    Rely on the robust reaction performance, and high lot-to-lot consistncy of this product, thoroughly tested for a reproducible quality. Manufacturing and documentation are according to GMP (Good Manufacturing Practice) regulations.
Use FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl, for:
  • Hot start PCR and RT-PCR with high specificity, sensitivity and yield
  • Specific amplification of DNA fragments from various sources of DNA and for diverse down-stream applications
  • Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA Glycosylase
  • Manufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation
FastStart Taq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Heat activation: +95°C for 3-10 minutes (assay-dependent; recommendation is 10 minutes for full activation)
pH optimum: Approximately 9.0 (+25°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants).
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
Appearance: Clear to slightly opalescent, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Function test in PCR
(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Function test in qPCR using the LightCycler® System
(human genomic DNA, β-globin gene): Corresponds to reference
(plasmid DNA, β-globin gene): Corresponds to reference
(reverse transcribed cDNA, PBGD gene): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.