Taq DNA Polymerase, 5 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution
For further processing on its own or in a mixture as part of an IVD method only.
Taq DNA Polymerase, 5 U/μl material number and pack size: Material Number Pack Size 11147633103 custom fill 5 U/μL
The enzyme is supplied without reaction buffer.
- Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.
- For applications see Taq DNA Polymerase, GMP Grade, 5 U/μl
See Taq DNA Polymerase, GMP Grade, 5 U/μl
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v), pH approximately 8.0 at +4°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Function test in PCR using conventional blockcycler (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.