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Collagenase P

from Clostridium histolyticum

For further processing only.

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Order information
Collagenase P material number and pack size:
Material Number Pack Size
11215809103 custom fill
Will be supplied as "Collagenase P from Clostridium histoly.". Unit of measure is "G".
The product is prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.
Appearance: Light brown to brown lyophilized substance
Activity (+25°C; with PZ-Pro-Leu-Gly-Pro-D-Arg ): ≥1.5 U/mg,lyophilized material

Contaminants expressed as percentage of collagenase P activity:
Clostripain: no limit (U/mg lyophilized material)
Proteases (azocoll): no limit (U/mg lyophilized material)
Trypsin (with BAEE): no limit (U/mg lyophilized material)
Performance test with pancreatic cells:corresponds
Stability: At + 2°C to + 8°C: within specification range for 12 months; stored dry and protected from light.