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Liberase MNP-S GMP Grade

Blended Proteolytic Enzyme for Tissue Dissociation. For Further Processing only.

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Order information
Liberase MNP-S GMP Grade material number and pack size:
Material Number Pack Size
06297790001 5 mg
Will be supplied as "Liberase MNP-S GMP Grade". Unit of measure is "piece".
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Overview
Liberase MNP-S is a highly purified enzyme blend for tissue dissociation where high purity, consistent quality, low levels of bacterial endotoxins and a sterile product are required for reproducible high cell yield and viability.
Liberase MNP-S contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other and with a medium concentration of highly purified thermolysin, a neutral protease isolated from Bacillus thermoproteolyticus. The blend is manufactured in accordance with Good Manufacturing Practice (GMP).
  • Maximum cell yield, viability, and functionality
    Use of high-specific-activity blends with reduced clostripain, trypsin, and endotoxin content maximizes yield of viable, functional cells.
  • Reduced contamination risk
    Manufactured free of any mammalian- or avian-derived raw materials, avoiding the risk of transmission of animal diseases.
  • High experimental reproducibility
    High lot-to-lot consistency due to optimized enzyme fermentation and purification processes results in increased experimental reproducibility, ultimately saving time and resources.
  • Flexible enzyme portfolio
    Custom sizes and blends are available upon request to better serve your unique application needs.
  • Specific residual assay
    Highly sensitive assay developed specifically for detection of Liberase enzyme verifies removal from your final product.
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Application
Use Liberase MNP-S, GMP Grade for in vitro enzymatic dissociation of tissue, to isolate single cells from a broad range of tissue types. In particular intended for use in isolation of tissue based stem cells and chondrocytes from cartilage.
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Specification
Appearance: White lyophilized cake
Total protein (Mass/bottle): 5.5-8.3 mg (target: 6.9 mg protein)
Collagenase I b, c part of collagenase I: ≤40 %area
Collagenase I content: 2.2-3.4 mg (target: 2.8 mg protein)
Collagenase II content: 1.5-2.3 mg/bottle (target: 1.9 mg protein)
Neutral Protease content: 1.8-2.6 mg (target 2.2 mg)
Activity (Wünsch, calculated): 19-32 U/bottle
Activity (Neutral Protease, calculated): 19,140 - 37,000 U/bottle
Ratio Collagenase II/Total Collagenase (HPLC): 0.3-0.5
Endotoxin: ≤15 EU/mg protein
Stability: At -15 to -25°C within specification range for 36 months.