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Hepes

crystalline powder

For further processing only.

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Order information
Hepes material number and pack size:
Material Number Pack Size
10172944103 custom fill
Will be supplied as "Hepes". Unit of measure is "kg".
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Overview
Buffer for diagnostic tests, such as amylase test.
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
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Application
Use Hepes as a buffer in reagents.
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Specification
Nomenclature: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
Formula: C8H18N2O4S
Molecular weight: 238.3 D
Suggested pH range: 6.8-8.2
Appearance: White crystalline powder
Solubility: Clear, colorless solution in water (c=1 mol/L)
pK value: 7.21-7.41 (+37°C); 7.45-7.65 (+20°C)
Melting range: +207 to +213°C
Hepes (alcalimetric): ≥97%
Hepes (from N): ≥97%
N (elementary analysis): ≥11.4%
Thin layer chromatography (TLC): Chromatographically homogeneous
A260 (against water): ≤0.050
A405 (against water): ≤0.030
Cl (chloride meter): ≤0.04%
Hepes mA/min (Purity check, α-amylase contamination): ≤0.1
Exclusion of skin contact and contamination with salvia: Corresponds to specification.
Stability: At +15 to +25°C within specification range for 24 months.