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Pyrophosphatase, rec., GMP Grade

from yeast, expressed in E. coli, solution

For further processing only.

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Pyrophosphatase, rec., GMP Grade material number and pack size:
Material Number Pack Size
08140677103 20 mL
Will be supplied as "Pyrophosphatase, rec., GMP Grade, 20 ml". Unit of measure is "piece". 
Pyrophosphatase is an enzyme, which hydrolyzes inorganic pyrophosphate.
Use fit-for-purpose Pyrophosphatase for manufacturing of therapeutic mRNA:
  • Pyrophosphatase is produced in antibiotic-free and animal-free conditions
  • QC-release testing includes extended impurity testing e.g. host-cell DNA, host cell proteins, endotoxin, bioburden, heavy metals
Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.
Cofactor: Mg2+ is required for enzymatic activity
Pyrophosphatase is expressed with an N-terminal His-Tag.
Molecular weight: 63 kD
Appearance: Clear, colorless to slightly yellowish solution
Volume activity: ≥ 600 U/mL
Protein (abs. 280nm): 1.8-2.2 mg/mL
Purity (SDS-PAGE): ≥ 95 %
Unspecific endonucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA) 16 h/37°C): not detectable
RNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Bioburden: ≤ 10 CFU/mL
E. coli host cell DNA: ≤ 100 pg/mg Protein
E. coli host cell protein: ≤ 50 ppm
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -15 to -25°C within specification range for 18 months.