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Carboxypeptidase B, recombinant

from rat pancreas, expressed in Pichia pastoris, solution

For further processing only.

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Order information
Carboxypeptidase B, recombinant material number and pack size:
Material Number Pack Size
03358682103 0.03 MU
Will be supplied as "CpB rec.". Unit of measure is "MU".
Carboxypeptidase B, recombinant, is intended to use in highly regulated production processes at pharmaceutical companies.
Carboxypeptidase B is a widely used metalloprotease, typically isolated from pancreas of different animals, that specifically releases arginine and lysine from the C-terminus of peptides and proteins. Roche has chemically synthesized a gene encoding for the amino acid sequence of the rat Carboxypeptidase B and has transformed the gene into the expression host Pichia pastoris, which expresses the recombinant Carboxypeptidase B as active protease with identical properties compared to the native Carboxypeptidase B.The production process is validated resulting in a very high lot-to-lot consistency.
  • Minimize the risk of virus contamination and the risk of animalrelated cross-contamination.
  • Rely on high purity. Minimize the risk of host cell protein contamination in your final product.
  • Increase the safety of your production processes with robust and reproducible performance and high lot-to-lot consistency.
Use the animal component-free and DIN EN ISO 13485-manufactured Carboxypeptidase B, recombinant, as critical raw material for the production of active pharmaceutical ingredients (API), i.e., insulin.
Molecular weight: 34.6 kD
The product is manufactured according to DIN EN ISO 13485 .
No animal-derived products are used in the fermentation, purification and final formulation.
Appearance: Clear, colorless to slightly yellowish solution
Storage buffer: Tris/HCl, 33 mmol/L; ZnCl2, 0.1 mmol/L
pH (at +25°C): 7.5-8.5
Activity (with hippurylarginine): ≥400 U/mL
Total activity: 30 kU ±15%
Specific activity: ≥210 U/mg
Protein: 2.8±0.5 mg/mL
Purity (RP-HPLC): ≥85% according to master lot
Bioburden: ≤50 CFU/mL
Trypsin (with chromozym): ≤0.005%
Shipping conditions: Dry ice
Stability: At -15 to -25°C within specification range for 12 months.