• Broad spectrum

    of products for

    your industry

Malate Dehydrogenase, IFCC Quality

from pig heart, solution

For further processing only.

expand | collapse
Order information
Malate Dehydrogenase, IFCC Quality material number and pack size:
Material Number Pack Size
10417726103 custom fill
Will be supplied as "MDH, IFCC-quality, Pig Heart (Mitochon.)". Unit of measure is "MU".
Dehydrogenase that catalyzes the interconversion of malate to oxaloacetate.
Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.
Nomenclature: L-malate:NAD+ oxidoreductase
Molecular weight: 70 kD
Isoelectric point: 6.1-6.4
Michaelis constants (Phosphate buffer, 95 mmol/L, pH 8.3, +25°C):
L-malate: 4.0 x 10-4 mol/L
L-tartrate: 9.0 x 10-3 mol/L
meso-tartrate: 1.2 x 10-3 mol/L
oxaloacetate: 3.3 x 10-5 mol/L
Inhibitors: Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Activators: Phosphate, arsenate, Zn2+
pH optimum: 7.5 (see figure for product Cat. No. 10 267 155 103)
Temperature dependence: See figure for product Cat. No. 10 267 155 103
pH stability: 7.0-9.0 (see figure for product Cat. No. 10 267 155 103)
Thermal stability: Up to +40°C (see figure for product Cat. No. 10 267 155 103)
Specificity: L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP.
Appearance: Clear, colorless solution in glycerol (50% (v/v))
pH value: 6.5-7.5
Specific activity (+30°C, oxaloacetate): ≥900 U/mg protein
Protein (Biuret): ≥10 mg/mL
Contaminants (expressed as percentage of Malate Dehydrogenase activity):
Aspartate aminotransferase (AST/GOT): ≤0.005
Alanine aminotransferase (ALT/GPT): ≤0.005
Glutamate dehydrogenase: ≤0.003
Reagent blank for determination of aspartate aminotransferase (AST/GOT): ≤0.009 (A334/10 minutes)
SVD free: Corresponds to specification
pH 5.5 treatment (for at minimum 30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.