• AptaTaq DNA Polymerase


    Hot start redefined

AptaTaq DNA Polymerase gives you the advantages of a hot start enzyme plus speed and robustness

The enzyme aptamer-oligonucleotide mixture is a reversible, temperature-dependent hot start system. The aptamer acts as a molecular switch, changing its temperature-dependent tertiary structure. Dropping the temperature below +55°C shuts off the polymerase activity, while temperatures above +60°C fully activate the enzyme.

Benefits

  • Reduce time to result 
    Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols. 
  • Maximize specificity, sensitivity, and yield 
    Achieve reliable amplification of your target DNA from various sources, such as genomic DNA, cDNA, and plasmids. 
  • Simplify PCR setup
    Store these highly stable polymerase for up to 1 month at +2 to +8°C and set up your hot start PCR reaction at room temperature. 
  • Obtain consistent results
    Rely on our standardized manufacturing processes, including extensive QC release testing and thus, high lot-to-lot consistency, providing the perfect basis for (IVD) kit manufacturers and end users. 
  • 50 U/μl, glycerol-free
    Prepare stable amplification mixes in dry format. Use this formulation to produce dry amplification mixes stable at room temperature.

Aptamer-based hot start mechanism

Hot start mechanism:

The enzyme aptamer-oligonucleotide mixture is a reversible, temperature-dependent hot start system. Similar to antibody-based methods, the enzyme is activated by heating. However, in contrast to antibodies, the aptamer oligonucleotides are synthetically manufactured and less temperature sensitive, reducing the risk of contamination and allowing storage at room temperature. Enzyme inactivation is achieved by a tight bond of the aptamer-oligonucleotide to the polymerase at lower temperatures. The aptamer acts as a molecular switch, changing the temperature-dependent tertiary structure. Dropping the temperature below +55°C shuts off the polymerase activity; temperatures above +60°C fully activate the enzyme.

AptaTaq-Hot-Start-Mechanism

Hot start mechanism