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KAPA3G Plant PCR Kit

mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer with dNTPs

For further processing only.

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Order information
KAPA3G Plant PCR Kit material number and pack size:
Material Number Pack Size
08041091001 1000 reactions
Will be supplied as "KAPA3G Plant PCR Kit". Unit of measure is "piece".
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Overview
PCR kit for processing crude samples or for direct PCR from crushed plant samples; Kit contains a 3rd gen. Taq mutant for maximum PCR inhibitor tolerance.
The kit contains hot start DNA polymerase, a reaction buffer including dNTP's, and separate Magnesium solution to optimize the amplification reaction.
  • Perform direct PCR from a variety of plant species.
    Use KAPA3G Plant PCR Kit for samples such as leaf discs, seeds and crude plant extracts.
  • Streamline your workflow and reduce turnaround time.
    Efficiently amplify long and difficult targets from all crude sample types.
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Application
The KAPA3G Plant PCR Kit is ideally suited for:
  • Amplification of fragments up to 5 kb in size from purified plant DNA, extracted with commercial kits
  • Direct PCR from leaf discs, seed samples, and other plant tissue types
  • PCR from crude plant DNA extracts, prepared from leaf and/or seed material.
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Specification
The KAPA3G Plant PCR Kit is designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR).
Volume activity: 2.5 U/μL  
Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids
(E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 0.4 ng DNA): Corresponds to specification