For further processing only. Others KAPA2G Robust HotStart ReadyMix 2x KAPA2G Robust HotStart ReadyMix 3.9.1.1.1.5 2x concentrated 08041113001 KAPA2G Robust HotStart ReadyMix KAPA2G Robust HotStart ReadyMix Reagents, kits KK0013 12.5 mL Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "KAPA2G Robust HotStart Ready Mix". Unit of measure is "piece". 2x concentrated, ready-to-use antibody-mediated hot start PCR mix, containing KAPA2G DNA Polymerase in an optimized concentration for amplification of crude sample types and/or AT- or GC-rich targets. en Simplify your workflow by working with crude samples.KAPA2G Robust shows high tolerance to inhibitor carry-over and crude sample PCR (e.g. FFPE) Use the same protocol for difficult targets. Work with GC- and AT-rich targets and shorten optimization time of your assays en Antibody-mediated hot start DNA master mix with improved inhibitor resistance containing a 2nd generation Taq mutant. en Amplification of DNA fragments up to 3 kb in PCR assays from a wide variety of templates. Particularly suited for: Assays which perform poorly with wild-type Taq Amplification of DNA fragments with high GC- or AT-content Amplification from template samples that contain PCR inhibitors (e.g. salts, urea, SDS, ethanol, EDTA) at concentrations that inhibit wild-type Taq Amplification from crude samples, e.g. colony PCR, or PCR from crude extracts, such as those prepared using KAPA Express Extract. en Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C. Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C. Tests for the presence of contaminating nucleic acids (E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification (human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification Performance test (≥ 0.1 ng human genomic DNA): Corresponds to specification en