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KAPA2G Fast HotStart ReadyMix

2x concentrated

For further processing only.

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Order information
KAPA2G Fast HotStart ReadyMix material number and pack size:
Material Number Pack Size
08041172001 12.5 mL
Will be supplied as "KAPA2G Fast HotStart Ready Mix". Unit of measure is "piece".
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Overview
Antibody-mediated hot start DNA master mix for fast PCR containing a 2nd generation Taq mutant.
2x concentrated, ready-to-use antibody-mediated hot start PCR mix, containing KAPA2G DNA Polymerase in an optimized concentration for fast amplification protocols.
  • Save valuable time.
    Reach extension times as low as 1 sec/kb and reduce PCR reaction times by up to 75%.
  • Work with difficult templates.
    Master mix is designed to work with AT- and GC-rich targets.
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Application
Fast amplification of DNA fragments up to 3 kb:
  • Fast PCR
  • Routine PCR
  • Genotyping
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Specification
KAPA2G Fast HotStart DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, it provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids
(E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 1 ng human genomic DNA): Corresponds to specification