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Ala-Gln Bio

Test kit for the Cedex Bio Analyzer.

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Order information
Ala-Gln Bio material number and pack size:
Material Number Pack Size
08056978001 200 tests
Unit of measure is "piece".
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Overview
Kit for determination of the dipeptide Ala-Gln (GlutaMAX).
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Application
Glutamine is an essential nutrient required in cell cultures and microbial fermentation. Under culture conditions, glutamine has the disadvantage of fast degradation, producing cell-toxic ammonia. The dipeptide Ala-Gln is heat-stable and shows no spontaneous breakdown. Living cells in a culture are able to gradually release glutamine from the Ala-Gln dipeptide by secretion of peptidases. Depending on the number of cells and on the intensity of their metabolism, more dipeptide has to be fed over the culture time to achieve permanently an optimal supply. For control of culture media and for monitoring in a culture process, the Ala-Gln concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.
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Specification
Method: Enzymatic photometric assay using hydrolysis by peptidase and deamination of glutamine by glutaminase.
Recovery: ± 15%