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Alkaline Phosphatase, recombinant, highly active, carbohydrate reduced

from calf intestine, expressed in Pichia pastoris, solution

For further processing only.

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Order information
Alkaline Phosphatase, recombinant, highly active, carbohydrate reduced material number and pack size:
Material Number Pack Size
03535452103 custom fill
Will be supplied as "AP, highly active, recombinant, CR". Unit of measure is "g".
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Application
Marker enzyme for preparation of antibody- / antigen- enzyme conjugates incorporated in highly sensitive immunoassay reagents for colorimetric, fluorimetric and luminometric detection. Recommended for conjugation via amino groups.
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Specification
IEP (IEF, CE): 5.2-6.0
MALDI-TOF MS:
Total molecular weight: 111 kD
Molecular weight protein: 104 kD (= 94%)
Molecular weight carbohydrates: 6.5 kD (= 6%)
Accessible N-glycosylation sites: 2/subunit
Branching type: Reduced branched type GlcNAc, Mannose no NeuAc detected
O-glycosylation sites: Not detected
Number of isoenzymes:
Present: 1 (MS)
Based on protein: 1 (MS)
Appearance: Clear, colourless solution in NaCl, 3 mol/L; MgCl2, 5 mmol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L, pH approximately 7.6
pH value: 7.0-8.0
Protein (A280; 1 mg/mL=1; against water): 20±1 mg/mL
Specific activity (+37°C, 4-NPP): ≥ 7000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 5-13 mol/mol
Carbohydrates, n=2: ≤7%
Stability: At +2 to +8°C within specification range for 12 months.