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Alkaline Phosphatase Mutein, recombinant

from calf intestine, expressed in Pichia pastoris, lyophilizate

For further processing only.

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Order information
Alkaline Phosphatase Mutein, recombinant material number and pack size:
Material Number Pack Size
04781007103 custom fill
Will be supplied as "AP-Mutein, rec.". Unit of measure is "g".
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Overview
In addition to the AP Mutein, recombinant the lyophilizate contains a mixture of proteins from the Pichia system supporting the interference elimination. AP Mutein is lyophilized from a solution containing in NaCl, 0.2 mol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L; MgCl2, 1 mmol/L; raffinose, 50% (w/v); pH approximately 7.6. Production is established according to the procedures of the active enzyme.
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Application
Use Alkaline Phosphatase Mutein (AP Mutein) to eliminate human serum derived AP directed assay interferences.
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Specification
Appearance: White to yellowish lyophilizate
Solubility: Clear to light yellowish solution in 50% glycerol solution (c=10 mg/mL)
pH value: 7.0-8.0
Protein (A280, 1 mg/mL=1, against water): ≥0.2 mg protein/mg lyophilizate
Specific activity (+37°C, 4-NPP): ≤10 U/mg protein
SDS-gel (qualitative comparison of the gel bands in reference to the bands of a standard): Corresponds to specification
Stability: At +2 to +8°C within specification range for 24 months.