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Alkaline Phosphatase, EIA Grade

from calf intestine, solution

For further processing only.

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Order information
Alkaline Phosphatase, EIA Grade material number and pack size:
Material Number Pack Size
10556602103 custom fill
Will be supplied as ''Phosphatase, Alkaline, Calf Intestine''. Unit of measure is ''g''.
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Application
Marker enzyme for preparing antibody- / antigen-enzyme conjugates incorporated in immunoassay reagents for colorimetric, fluorimetric and luminometric detection.
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Specification
Nomenclature: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Molecular weight: ≥57 kD
Inhibitors: Pi, metal chelating agents, divalent heavy metal ions (e.g., Be2+, Zn2+), many amino acids (e.g., L-phenylalanine, L-tryptophan, L-cysteine), iodosobenzoate, iodoacet-amide.
Activators: Mg2+, Co 2+, Mn2+
pH optimum: 9.8
pH stability: 8.0
Thermal stability: Up to +40°C
Appearance: Clear, colourless solution in NaCl, 3 mol/L; MgCl2, 1 mmol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L
pH value: 7.0-8.0
Protein (A280, 1 mg/mL=1, against water): ≥10 mg/mL
Specific activity (+37°C, 4-NPP): ≥3,000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 8-16 mol/mol
Carbohydrates, n=2: No limit
Molecular weight: ≥57,000 D
NH4: ≤30 ppm
pH 5.5 treatment (30 minutes): Corresponds to specification
Origin of bovine intestine: NZL
Stability: At +2 to +8°C within specification range for 15 months.