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Expand High Fidelity PCR Buffer

10x conc., without MgCl2

For further processing only.

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Order information
Expand High Fidelity PCR Buffer material number and pack size:
Material Number Pack Size
05917123103 1 mL (1 piece contains 1 mL)
Will be supplied as "Exp.HF Buff. 10x w/o MgCl2 MPB". Unit of measure is "piece".
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Overview
Standard reaction buffer without MgCl2 for optimization of MgCl2 concentration in PCR using the Expand High Fidelity PCR System.
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Application
Use this buffer together with Expand High Fidelity PCR System. whenever the amplification of difficult targets requires a MgCl2 concentration that needs to be individually optimized.
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Specification
Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 220 mmol/L; pH approximately 8.9 at +25°C
Unspecific endonucleases (λDNA and MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.