• Broad spectrum

    of products for

    your industry

FastStart PCR Buffer

10x conc., with 20 mM MgCl2

For further processing only.

expand | collapse
+-
Order information
FastStart PCR Buffer material number and pack size:
Material Number Pack Size
12161516103 custom fill 10x conc., with 20 mM MgCl2
12161516103: Will be supplied as ''PCR buffer (10X) w MgCl2''. Unit of measure is ''mL''.
+-
Overview
Standard reaction buffer for PCR using FastStart Taq DNA Polymerase.
+-
Application
Use this buffer together with FastStart Taq DNA Polymerase. For applications refer to FastStart Taq DNA Polymerase, 5 kU, GMP Grade.
+-
Specification
Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 50 mmol/L; KCl, 100 mmol/L; MgCl2, 20 mmol/L; pH approximately 8.3 at +25°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR (50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.