FastStart PCR Buffer
10x conc., with 20 mM MgCl2
For further processing only.
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FastStart PCR Buffer material number and pack size: Material Number Pack Size 12161516103 custom fill (10x conc., with 20 mM MgCl2)
- Standard reaction buffer for PCR using FastStart Taq DNA Polymerase.
- Use this buffer together with FastStart Taq DNA Polymerase. For applications refer to FastStart Taq DNA Polymerase, 5 kU, GMP Grade.
Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 50 mmol/L; KCl, 100 mmol/L; MgCl2, 20 mmol/L; pH approximately 8.3 at +25°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR (50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.