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Terminal Transferase Reaction Buffer

5x concentrated

For further processing only.

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Order information
Terminal Transferase Reaction Buffer material number and pack size:
Material Number Pack Size
06280927103 1 mL
Will be supplied as "React buffer, term.transf., Sar. 1 mL". Unit of measure is "piece".
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Overview
Reaction buffer for Terminal Transferase, recombinant.
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Application
Use Terminal Transferase Reaction Buffer for optimal results with Roche's Terminal Transferase, recombinant.
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Specification
Appearance: Clear, colorless solution
Contents: Potassium cacodylate, 1 mol/L; Tris/HCl, 125 mmol/L; BSA, 1.25 mg/mL; pH 6.6±0.1 at +20°C
pH value (+20°C): 6.50-6.70
Unspecific endonucleases (MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Performance test (tailing of a 30-mer oligonucleotide): Corresponds to specification
Stability: At -15 to -25°C within specification range for 18 months.