Protector RNase Inhibitor
from rat lung, expressed in E. coli
For further processing only.
Protector RNase Inhibitor material number and pack size: Material Number Pack Size 03335429103 custom fill
- Protector RNase Inhibitor is useful in any application where RNA degradation by RNases is critical.
- Use Protector RNase Inhibitor to:
- Protect RNA in cDNA synthesis reactions, in vitro transcription/translation systems, in vitro RNA synthesis, and in vitro virus replication
- Protect RNA during RNA isolation and purification
- Produce RNase activity-free antibodies
Protector RNase Inhibitor is a protein of 50 kD which inhibits enzymatic activity of RNases by noncovalently binding to the active site.
Activity: A minimum of 1 mmol/L DTT is required to maintain the inhibitor in its active state; a pH value between 5.0 and 9.0 is recommended (isoelectric point is at pH 4.7).
Inactivation: At temperatures >+65°C or under severe denaturing conditions the inhibitory activity disappears. Appearance: Clear, colorless solution
Storage buffer: Hepes-KOH, 20 mmol/L; KCl, 50 mmol/L; DTT, 8 mmol/L; glycerol, 50% (v/v); pH approximately 7.6
Volume activity: ≥40 U/μL
Specific activity: ≥80 kU/mg protein
Unit definition: One unit Protector RNase Inhibitor is defined as the amount which inhibits 50% of the activity of 5 ng RNase A (activity measured according to Blackburn by inhibition of hydrolysis of cyclic cytidine-monophosphoric acid).
Purity (SDS PAGE): ≥95%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 400 U after 1 hour incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 400 U after 1 hour incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 400 U after 1 hour incubation at +37°C.
Ribonuclease activity after thermal inactivation of inhibitor (10 minutes at +65°C, MS2 RNA): Not detectable in up to 160 U after 1 hour incubation at +37°C.
Function test in RT-PCR (Titan One Tube RT-PCR Kit with control RNA): Corresponds to reference
Function test in real-time RT-qPCR using the LightCycler® instrument (PBGD gene fragment from RNA standards): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.
62:NOTICE TO PURCHASER: DISCLAIMER OF LICENSE
This product is optimized for use in the polymerase chain reaction (PCR) covered by patents owned by F. Hoffmann-La Roche Ltd (“Roche”). No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of this product. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Roche, Applied Biosystems or other licensed suppliers’ reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. Diagnostic purposes require a license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.