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5-Aminoallyl-dUTP

powder, lithium salt

For further processing only.

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Order information
5-Aminoallyl-dUTP material number and pack size:
Material Number Pack Size
11115502103 custom fill
Will be supplied as "5-Allylamino-2'-deoxy-UTP, Lithium Salt". Unit of measure is "μmol".
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Overview
Aminoallyl-modified nucleotides are used for efficient target labeling and a wide variety of dyes and haptens for subsequent post-labeling reactions.
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Application
5-Aminoallyl-16-dUTP replaces dTTP in labeling reactions using:
  • Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • T4 and T7 DNA polymerase
  • Taq DNA polymerase and reverse transcriptases
Lacking bulky dye groups, aminoallyl-modified nucleotides can be incorporated at extremely high and reproducible levels. Reaction of the amine-modified nucleic acid with an excess of amine-reactive dye or hapten results in high and consistent labeling efficiencies, regardless of the dye or hapten chosen. The two-step labeling method eliminates the need to optimize the labeling reaction.
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Specification
Nomenclature: 5-Aminoallyl-2'-deoxy-uridine-5'-triphosphate
Formula: C12H16N3O14P3Li4
Molecular weight: 547 D
Appearance: White powder
5-Aminoallyl-dUTP (1 μmol ≙ 9.73 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): ≥90%
Purity (HPLC, including two prepeaks): ≥75 area%
Stability: At -15 to -25°C within specification range for 12 months. Store dry.