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7-Deaza-2'-dGTP

lithium salt, 10 mM

For further processing only.

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Order information
7-Deaza-2'-dGTP material number and pack size:
Material Number Pack Size
10987891103 100 mL (1000 μmol)
Will be supplied as ''7-Deaza-2'-deoxy-GTP, Di-Li''. Unit of measure is ''μmol''.
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Overview
Use 7-Deaza-dGTP instead of dGTP in primer-extension reactions or PCR and receive a better resolution of GC-rich regions.
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Application
Use 7-Deaza-dGTP as a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
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Specification
Nomenclature: 7-Deaza-2'-deoxy-guanosine-5'-triphosphate
Formula: C11H17N4O13P3
Molecular weight: 506.2 D
Appearance: Clear, colorless solution
pH value: 6.8-7.2
7-Deaza-2'-dGTP (1 μmol ≙ 13.4 A259 units): 10.0-11.0 mmol/L
7-Deaza-2'-dGTP (HPLC): ≥95 area%
7-Deaza-2'-dGDP (HPLC): ≤4 area%
A250/A260: 0.84±0.04
A280/A260: 0.65±0.03
A290/A260: 0.53±0.03
Stability: At -15 to -25°C within specification range for 30 months.