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ddATP, Sequencing Grade

sodium salt, 10 mM

For further processing only.

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Order information
ddATP, Sequencing Grade material number and pack size:
Material Number Pack Size
12158175103 100 ml 1,000 μmol
Will be supplied as "Dideoxy-ATP, Na, Sequencing Grade, Solution". Unit of measure is "μmol".
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Overview
For consistent performance in sequencing applications and primer extension reactions, use dideoxyribonucleotides from the leading manufacturer of nucleotides.
ddATP, Sequencing Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The pH is adjusted to match conditions for amplification.
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Application
ddATP, Sequencing Grade, in dNTP mixes acts as chain terminator in sequencing and primer extension reactions. High purity and proven manufacturing processes produce a product ideal for the manufacturing of in vitro diagnostics.
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Specification
Nomenclature: 2',3'-Dideoxy-adenosine-5'-triphosphate
Formula: C10H16N5O11P3
Molecular weight: 475.2 D
Appearance: Clear, colorless solution
pH value: 8.1-8.5
ddATP (1 μmol ≙ 15.3 A260 units, pH 7.0): 10.0-11.0 mmol/L
ddATP (HPLC): ≥98 area%
ddADP (HPLC): ≤1.5 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.76±0.02
A280/A260: 0.15±0.01
A290/A260: ≤0.02
Stability: At -15 to -25°C within specification range for 24 months.