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ddTTP, Sequencing Grade

sodium salt, 10 mM

For further processing only.

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Order information
ddTTP, Sequencing Grade material number and pack size:
Material Number Pack Size
12158205103 100 mL (1,000 μmol)
Will be supplied as "Dideoxy-TTP, Na, Sequencing Grade, Solution". Unit of measure is "μmol".
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Overview
For consistent performance in sequencing applications and primer extension reactions, use dideoxyribonucleotides from the leading manufacturer of nucleotides.
ddTTP, Sequencing Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The pH is adjusted to match conditions for amplification.
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Application
ddTTP, Sequencing Grade, in dNTP mixes acts as chain terminator in sequencing and primer extension reactions. The high purity and carefully designed manufacturing process are ideally suited for the manufacturing of in vitro diagnostics.
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Specification
Nomenclature: 2',3'-Dideoxy-thymidine-5'-triphosphate
Formula: C10H17N2O13P3
Molecular weight: 466.2 D
Appearance: Clear, colorless solution
pH value: 8.1-8.5
ddTTP (1 μmol ≙ 8.4 A260 units, pH 7.0): 10.0-11.0 mmol/L
ddTTP (HPLC): ≥98 area%
ddTDP (HPLC): ≤1.5 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.60±0.02
A280/A260: 0.83±0.03
A290/A260: 0.31±0.02
Stability: At -15 to -25°C within specification range for 24 months.