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dUTP, PCR Grade

sodium salt, 100 mM

For further processing only.

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Order information
dUTP, PCR Grade material number and pack size:
Material Number Pack Size
11889532103 100 ml 10,000 μmol
04631145103 20 ml 2,000 μmol
11889532103: Will be supplied as ''dUTP, Na, Solution (PCR Grade)''. Unit of measure is ''μmol''.
04631145103: Will be supplied as ''dUTP PCR Grade, Sodium Solution, 20 mL''. Unit of measure is ''μmol''.
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Overview
GMP-manufactured dNTP's from the leading manufacturer of nucleotides for outstanding, consistent performance in amplification reactions.
dUTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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Application
dUTP, PCR Grade, is designed for amplification reactions where high-quality reagents are required, such as for in vitro diagnostics. Avoid DNA carryover contamination between PCRs that can be a source of false positives.
To decontaminate PCR and RT-PCR mixes, dUTP is incorporated in place of dTTP into the PCR product. Reaction mixes are then pre-treated with Uracil-DNA Glycosylase (UNG) before amplification to degrade contaminating dUTP-containing PCR products.
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Specification
Nomenclature: 2'-Deoxy-uridine-5'-triphosphate
Formula: C9H15N2O14P3
Molecular weight: 468.2 D
Appearance: Clear, colorless solution
pH value: 8.1-8.5
dUTP (1 μmol ≙ 9.9 A260 units, pH 7.0): 100-110 mmol/L
dUTP (high resolution HPLC method): ≥99 area%
dUDP (HPLC): ≤0.9 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.74±0.02
A280/A260: 0.38±0.02
A290/A260: 0.04±0.01
Function test in RT-PCR (RNA, human dystrophin, and mouse β-actin gene): Corresponds to specification
Stability: At -15 to -25°C within specification range for 42 months.