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dTTP, PCR Grade

sodium salt, 100 mM

For further processing only.

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Order information
dTTP, PCR Grade material number and pack size:
Material Number Pack Size
04631137103 20 mL (2,000 μmol)
11889559103 100 mL (10,000 μmol)
11889559103: Will be supplied as "dTTP, Na, Solution (PCR Grade)". Unit of measure is "μmol".
04631137103: Will be supplied as "dTTP PCR Grade, Sodium Solution, 20 mL". Unit of measure is "μmol".
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Overview
GMP-manufactured dNTP's from the leading manufacturer of nucleotides for outstanding, consistent performance in amplification reactions.
dTTP, PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
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Application
dTTP, PCR Grade, is designed for amplification reactions where high-quality reagents are required, such as for in vitro diagnostics.
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Specification
Nomenclature: 2'-Deoxy-thymidine-5'-triphosphate
Formula: C10H17N2O14P3
Molecular weight: 482.2 D
Appearance: Clear, colorless solution
pH value: 8.1-8.5
dTTP (1 μmol ≙ 9.5 A267 units, pH 7.0): 100-110 mmol/L
dTTP (high resolution HPLC method): ≥99 area%
dTDP (HPLC): ≤0.9 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.64±0.02
A280/A260: 0.74±0.02
A290/A260: 0.24±0.02
Function test in RT-PCR (RNA, human dystrophin, and mouse β-actin gene): Corresponds to specification
Stability: At -15 to -25°C within specification range for 42 months.