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MgCl2 Stock Solution

25 mM

For further processing only.

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Order information
MgCl2 Stock Solution material number and pack size:
Material Number Pack Size
11600788103 custom fill
11600770103 1 mL
11600770103: Will be supplied as "MgCl2-Solution 25mM MPB". Unit of measure is "piece".
11600788103: Will be supplied as "MgCl2-Solution 25mM/Bulk". Unit of measure is "mL".
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Overview
Stock Solution for the preparation of optimized PCR reaction mixes or master mixes.
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Application
Use this MgCl2 Stock Solution in combination with any PCR buffer without MgCl2 to optimize the magnesium concentration.
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Specification
Appearance: Clear, colorless solution
Contents: MgCl2, 25 mmol/L; pH approximately 8.3 at +20°C
Unspecific endonucleases (λDNA and MWM III DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activitiy (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (0.01 ng λDNA, 0.5 kb lambda fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.