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Expand Long Template PCR Buffer 3

10x conc., with 27.5 mM MgCl2

For further processing only.

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Order information
Expand Long Template PCR Buffer 3 material number and pack size:
Material Number Pack Size
05420083103 1 mL
Will be supplied as "Expand LT PCR Buffer 3 (10x), 1 mL". Unit of measure is ''piece''.
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Overview
Reaction buffer 3 for PCR of fragments larger than 15 kb using the Expand Long Template PCR System.
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Application
Use this buffer together with Expand Long Template PCR System.
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Specification
Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 220 mmol/L; MgCl2, 27.5 mmol/L; DMSO, 20%; Tween 20, 1%; pH approximately 9.2 at +25°C
Unspecific endonucleases (λDNA and MWM II DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (200 ng human genomic DNA, 15 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.