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PCR Buffer

10x conc., with 15 mM MgCl2

For further processing only.

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Order information
PCR Buffer material number and pack size:
Material Number Pack Size
11974769103 1 mL
11974769103: Will be supplied as "PCR Buffer 10x 1 mL". Unit of measure is "piece".
11271326103: Will be supplied as "PCR Buffer 10x". Unit of measure is "mL".
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Overview
Standard reaction buffer for PCR using Taq DNA Polymerase.
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Application
Use this buffer together with Taq DNA Polymerase. For a list of applications, refer to Taq DNA Polymerase, GMP Grade, 5 U/μL.
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Specification
Appearance: Clear, colorless solution
Contents: Tris/HCl, 100 mmol/L; KCl, 500 mmol/L; MgCl2, 15 mmol/L; pH approximately 8.3 at +20°C
Unspecific endonucleases (λDNA and MWM III DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (0.01 ng λDNA, 0.5 kb lambda fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.