10x conc., without MgCl2
For further processing only.
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PCR Buffer material number and pack size: Material Number Pack Size 11600753103 1 mL (10x conc.) 11600761103 custom fill (10x conc.)
11600761103: Will be supplied as "PCR Puffer (10x) w/o MgCl2/Bulk". Unit of measure is "mL".
- Standard PCR reaction buffer without MgCl2 using Taq DNA Polymerase for individual MgCl2 optimization.
- Use this buffer without MgCl2 together with Taq DNA Polymerase, GMP Grade, 5 U/μl for amplification of difficult targets requiring a MgCl2 concentration that is optimized individually.
Appearance: Clear, colorless solution
Contents: Tris/HCl, 100 mmol/L; KCl, 500 mmol/L; pH approximately 8.3 at +20°C
Unspecific endonucleases (λDNA and MWM III DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activitiy (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (0.01 ng λDNA, 0.5 kb lambda fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.