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FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

For further processing only.

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Order information
FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl material number and pack size:
Material Number Pack Size
04659163103 5 kU
Will be supplied as "FastStart Taq DNA Pol. Ind. GMP Grd, 5KU". Unit of measure is "piece".
The enzyme is supplied without reaction buffer.
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Overview
Hot start Taq DNA Polymerase for highly specific and sensitive amplification using PCR.
  • Achieve high specificity, sensitivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
  • Obtain reliable results.
    Rely on the robust reaction performance, and high lot-to-lot consistncy of this product, thoroughly tested for a reproducible quality. Manufacturing and documentation are according to GMP (Good Manufacturing Practice) regulations.
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Application
Use FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl, for:
  • Hot start PCR and RT-PCR with high specificity, sensitivity and yield
  • Specific amplification of DNA fragments from various sources of DNA and for diverse down-stream applications
  • Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA Glycosylase
  • Manufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation
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Specification
FastStart Taq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Heat activation: +95°C for 3-10 minutes (assay-dependent; recommendation is 10 minutes for full activation)
pH optimum: Approximately 9.0 (+25°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants).
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
Appearance: Clear to slightly opalescent, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C
Volume activity: ≥5 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Function test in PCR
(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Function test in qPCR using the LightCycler® System
(human genomic DNA, β-globin gene): Corresponds to reference
(plasmid DNA, β-globin gene): Corresponds to reference
(reverse transcribed cDNA, PBGD gene): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.