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FastStart Taq DNA Polymerase, 100 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

For further processing only.

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Order information
FastStart Taq DNA Polymerase, 100 U/μl material number and pack size:
Material Number Pack Size
04433785103 custom fill
Will be supplied as "FastStart Taq DNA Pol. 100 U/μl". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
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Overview
Concentrated hotstart Taq DNA Polymerase for highly specific and sensitive amplification using PCR; suitable for preparation of dried amplification mixes.
  • Achieve high specificity, sensitivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
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Application
Use FastStart Taq DNA Polymerase, 100 U/μl, especially for:
  • Setup of PCR master mixtures, when highly concentrated components are requested
  • Preparation of stabilized dried-down formulations of reaction mixtures

For further applications, see FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl
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Specification
See FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50% (v/v); pH 9.0 at +25°C ±0.1
Volume activity: ≥100 U/μL
Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 15 U after 4 hours incubation at +65°C.
Function test in PCR
(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference
(human genomic DNA, 284 bp ApoE fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.