For further processing only. Others FastStart Taq DNA Polymerase 5 U uL from Thermus aquaticus BM expressed in E. coli solution FastStart Taq DNA Polymerase, 5 U/μl 3.6.17.2.9.1 from Thermus aquaticus BM, expressed in E. coli, solution 12161508103 Fast Start Taq DNA Polymerase FastStart Taq DNA Polymerase, 5 U/uL Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as ''Fast Start Taq DNA Polymerase''. Unit of measure is ''kU''. The enzyme is supplied without reaction buffer. Hot start Taq DNA Polymerase for highly specific and sensitive amplification using PCR. en Use FastStart Taq DNA Polymerase, 5 U/μl, for:Hot start PCR and RT-PCR with high specificity, sensitivity and yieldSpecific amplification of DNA fragments from various sources of DNA and for diverse down-stream applicationsLabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)The prevention of carryover contamination between PCR reactions in combination with dUTP and Uracil-DNA GlycosylaseManufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control) with requests for more stringent validation en Achieve high specificity, sensitivity, and yield. Prevent the extension of non-specifically bound primers using this hot start enzyme. en Appearance: Clear to slightly opalescent, colorless solutionStorage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Tween 20; 0.2% (v/v); glycerol, 50% (v/v); pH approximately 9.0 at +25°CVolume activity: ≥5 U/μLUnit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.Unspecific endonucleases (λDNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.Ribonucleases (MS2 RNA): Not detectable in up to 25 U after 1 hour incubation at +37°C.Function test in PCR(50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to reference(200 ng human genomic DNA, 284 bp ApoE fragment): Corresponds to referenceAnimal-derived additives: NoneStability: At -15 to -25°C within specification range for 18 months. en