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HawkZ05 DNA Polymerase, 40 U/μl

from Thermus species Z05, expressed in E. coli, solution

For further processing only.

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Order information
HawkZ05 DNA Polymerase, 40 U/μl material number and pack size:
Material Number Pack Size
05230349190 200 kU 40 U/μl
Will be supplied as "CMPNT HAWK Z05 DNA Polymerase 200KU 5 mL". Unit of measure is "piece".
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Overview
Reversible hot start DNA polymerase with reverse transcriptase activity for one-step RT-PCR.
  • Obtain increased detection sensitivity using this highly specific one- or two-step RT-PCR method.
  • Enjoy fast PCR cycling without RNA degradation by using this fast activating, reversible aptamer-based hot start method.
  • Control carryover contamination with a dUTP and Uracil-DNA Glycosylase treatment (UNG) compatible setup.
  • Rely on lot-to-lot consistency and full traceability with GMP manufacturing.
  • Simplify setup of PCR reactions using this stable aptamer hot start DNA polymerase at room temperature.
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Application
Apply HawkZ05 DNA Polymerase for:
  • Efficient, high temperature cDNA synthesis and subsequent DNA amplification of RNA templates
  • Use in multiplex PCR and qPCR applications that require high specificity, sensitivity, and yield
  • Incorporation of modified nucleotides for labeling of PCR products
  • Detection formats such as hydrolysis probes, hybridization probes and SYBR Green
  • Fast-cycling diagnostic applications and other routine amplification of low-copy targets
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Specification
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; EDTA, 0.1 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; Tween 20, 0.5% (v/v); glycerol, 50.0% (v/v); aptamer; pH approximately 8.0
Volume activity: 40-55 U/μL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.