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AptaTaq DNA Polymerase,
50 U/μl

from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution

For further processing only.

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Order information
AptaTaq DNA Polymerase,
50 U/μl material number and pack size:
Material Number Pack Size
05187605103 custom fill
Will be supplied as "AptaTaq DNA Polymerase, Glyc.-free, 50 U/uL". Unit of measure is "kU".
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Overview
Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity; lyo ready formulation for preparation of dried amplification mixes.
AptaTaq DNA Polymerase is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) providing hot start features. The concentrated formulation does not contain glycerol and is suitable for the preparation of dry amplification mix preparations.
  • Reduce time to result.
    Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols.
  • Maximize specificity, sensitivity, and yield.
    Achieve reliable amplification of your target DNA from various sources (e.g., genomic DNA, cDNA, plasmids).
  • Simplify PCR setup.
    Store these highly stable polymerase for up to 1 month at +2° to +8°C and setup your hot start PCR reaction at room temperature.
  • Obtain consistent results.
    Roche standardized manufacturing processes include extensive Quality Control release testing for high lot-to-lot consistency ideal for (IVD) kit manufacturers and end users.
  • Prepare stable amplification mixes in dry format.
    Use this formulation for producing dried-down amplification mixes stable at room temperature.
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Application
Apply AptaTaq DNA Polymerase for:
  • Fast PCR assays with no extra enzyme activation time and fast cycling protocols
  • Single- or multiplex PCR and qPCR applications requiring high specificity, sensitivity, and yield
  • RT-PCR
  • Difficult templates with secondary structures or GC-rich sequences
  • Formulation of dried-down amplification reagents
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Specification
AptaTaq DNA Polymerase is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase that lacks 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. The inherent stability of Taq DNA Polymerase is shown by the high storage stability in refrigerator and freezer (24 months at +2 to +8°C and -25 to -25°C). Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum for elongation: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg2+ (standard concentration, 1.5 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Volume activity: 55±5 U/μL
Glycerol content: ≤0.1% (v/v)
Aptamer concentration (HPLC): 35.75 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 24 months.
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Performance
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