Others AptaTaq DNA Polymerase 50 U uL AptaTaq DNA Polymerase, 50 U/μl For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only acc. to Art. 56 (3) and 3 no. 23 REACH Regulation. 3.6.17.2.1.9 from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution 05187605103 AptaTaq DNA Pol., Glycerol-free, 50 U/ul AptaTaq DNA Polymerase, 50 U/uL 07613336130865 Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "AptaTaq DNA Polymerase, Glyc.-free, 50 U/uL". Unit of measure is "kU". EC 2.7.7.7 en AptaTaq DNA Polymerase is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) providing hot start features. The concentrated formulation does not contain glycerol and is suitable for the preparation of dry amplification mix preparations. en Apply AptaTaq DNA Polymerase for:Fast PCR assays with no extra enzyme activation time and fast cycling protocolsSingle- or multiplex PCR and qPCR applications requiring high specificity, sensitivity, and yield RT-PCRDifficult templates with secondary structures or GC-rich sequencesFormulation of dried-down amplification reagents en Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity; lyo ready formulation for preparation of dried amplification mixes. en Reduce time to result. Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols. Maximize specificity, sensitivity, and yield. Achieve reliable amplification of your target DNA from various sources (e.g., genomic DNA, cDNA, plasmids). Simplify PCR setup. Store these highly stable polymerase for up to 1 month at +2° to +8°C and setup your hot start PCR reaction at room temperature. Obtain consistent results. Roche standardized manufacturing processes include extensive Quality Control release testing for high lot-to-lot consistency ideal for (IVD) kit manufacturers and end users. Prepare stable amplification mixes in dry format. Use this formulation for producing dried-down amplification mixes stable at room temperature. en Appearance: Clear, colorless solutionStorage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°CVolume activity: 55±5 U/μLGlycerol content: ≤0.1% (v/v)Aptamer concentration (HPLC): 35.75 μmol/L ±10%Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to referenceStability: At -15 to -25°C within specification range for 24 months. en