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AptaTaq DNA Polymerase LDx,
5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

For further processing only.

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Order information
AptaTaq DNA Polymerase LDx,
5 U/μl material number and pack size:
Material Number Pack Size
05884314103 custom fill 5 U/μl
Will be supplied as "AptaTaq DNA Polymerase LDx, 5 U/μL". Unit of measure is "kU".
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Overview
Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity in microbial testing.
AptaTaq DNA Polymerase LDx is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for applications detecting lowest levels of DNA.
  • Minimize risks from contaminating nucleic acids.
    AptaTaq DNA Polymerase LDx is extensively tested using ultra sensitive tests for contaminating nucleic acids from bacteria and fungi. Roche has developed a nucleic acid-free workflow with clearly defined, highly consistent manufacturing processes to offer a product with very low nucleic acid background.
  • Enjoy the benefits of the advanced AptaTaq hot start system.
    Use AptaTaq DNA Polymerase for additional benefits including speed, easy handling and consistent results.
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Application
Select AptaTaq DNA Polymerase LDx to perform microbial testing and other assays where the absence of contaminating bacterial, fungal, and/or human DNA is crucial. AptaTaq DNA LDx Polymerase is ideal for:
  • Fast PCR assays with no extra enzyme activation time and fast cycling protocols
  • Single- and multiplex PCR and qPCR applications that require high specificity, sensitivity, and yield
  • RT-PCR
  • Difficult templates with secondary structures or GC-rich sequences
  • Automated PCR workflows requiring high stability of the reaction mixtures during automated pipetting and prolonged handling at room temperature
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Specification
AptaTaq DNA Polymerase LDx is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase that lacks 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum for elongation: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg2+ (standard concentration, 1.5 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C
Volume activity: 5.5±0.5 U/μL
Aptamer concentration (HPLC): 3.58 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Tests for the absence of contaminating nucleic acids
(human genomic DNA, β-Globin fragment, ≤3 positve of 15 samples): Corresponds to specification
(LightCycler® UniTOOL ResoLight assay, detecting grampositive and gramnegative bacterial DNA and fungal DNA, <1.0 copy genomic DNA/20 U enzyme): Corresponds to specification
Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.