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EvoScript RNA Master

5x concentrated

For further processing only.

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Order information
EvoScript RNA Master material number and pack size:
Material Number Pack Size
07873468001 5 mL
Will be supplied as "EvoScript RNA Master, 5 mL". Unit of measure is "piece".
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Overview
True one-tube 5x hot start RT-PCR master mix for maximum ease-of-use. Ideal for high-specificity and high-precision one-step RT-qPCR reactions.
EvoScript RNA Master is a perfect master mix for convenient RT-qPCR reactions. It includes FastStart Taq DNA Polymerase and a designer polymerase for reverse transcription. It uses a sophisticated hot start system that includes chemical modification and aptamer-mediated hot start. This enables highly specific priming for both, reverse transcription and DNA amplification. The 1-vial composition is ideally suited for easy reaction assembly with just the addition of oligos and target RNA.
  • Save time.
    Just add primers, probes, and template to get started.
  • Be flexible.
    Use a variety of sample materials (e.g., whole blood, FFPE).
  • Achieve high sensitivity.
    Detect down to 10 copies of RNA target.
  • Ready for automation.
    Set up your reaction at room temperature.
  • Prevent carryover contamination.
    The mix is compatible with UNG protocols to prevent false positives.
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Application
Use EvoScript RNA Master for hot start one-step RT-qPCR reactions that require high specificity and performance. It is compatible with hydrolysis probe and hybridization probe formats.
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Specification
EvoScript RNA Master (5x) is stable at room temperature for 24 hours and at a final (1x) dilution including primers and probes for 4 hours. The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).
Appearance: Colorless solution
Function test RT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH assay): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.