Others AptaTaq DNA Polymerase 5 U uL AptaTaq DNA Polymerase, 5 U/μl For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only acc. to Art. 56 (3) and 3 no. 23 REACH Regulation. 3.6.17.2.1.12 from Thermus aquaticus BM, expressed in E. coli, solution 05457882103 AptaTaq DNA Polymerase, 5 U/ul AptaTaq DNA Polymerase, 5 U/uL 07613336130896 Reagents, kits custom fill 5 U/μL CustomBiotech product. Please contact your local representative. Will be supplied as "AptaTaq DNA Polymerase, 5 U/μL". Unit of measure is "kU". EC 2.7.7.7 en AptaTaq DNA Polymerase is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) providing hot start features. en Apply AptaTaq DNA Polymerase for: Fast PCR assays with no extra enzyme activation time and fast cycling protocolsSingle- and multiplex PCR and qPCR applications that require high specificity, sensitivity, and yieldRT-PCRDifficult templates, such as complex secondary structures or GC-rich sequencesAutomated PCR workflows requiring high stability of the reaction mixtures during automated pipetting and prolonged handling at room temperature en Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity. en Reduce time to result. Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols. Maximize specificity, sensitivity, and yield. Achieve reliable amplification of your target DNA from various sources (e.g., genomic DNA, cDNA, plasmids). Simplify PCR setup. Store these highly stable polymerase for up to 1 month at +2° to +8°C and set up your hot start PCR reaction at room temperature. en Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50.0% (v/v); pH approximately 8.0 at +4°C Volume activity: 5.5±0.5 U/μL Aptamer concentration (HPLC): 3.58 μmol/L ±10% Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C. Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference Stability: At -15 to -25°C within specification range for 12 months. en