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HawkZ05 Fast One-Step RT-PCR Master (Rox)

5x concentrated, 0.5% glycerol content

For further processing only.

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Order information
HawkZ05 Fast One-Step RT-PCR Master (Rox) material number and pack size:
Material Number Pack Size
06402232190 1 kit 5 ml master mix and 2 x 1 ml Manganese acetate
06402232190: Will be supplied as "KIT HAWKZ05 1STEP RTPCR LYO W/ROX 5mL". Unit of measure is "piece".
06402267190: Will be supplied as "KIT HAWKZ05 1STEP RTPCR LYO W/ROX 50mL". Unit of measure is "piece".
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Overview
Reversible hot start one-step RT-PCR master mix with fast RT-step and high stability; lyo ready formulation for preparation of dried amplification mixes.
HawkZ05 Fast One-Step RT-PCR Kit is supplied as a kit containing 2 vials of HawkZ05 Fast One-Step RT-PCR Master Mix and 2 vials of RMS Manganese Acetate (25 mM). The master mix contains a reference dye (FRET-ROX) to run assays on real-time PCR instruments which require Rox for quantitative analysis.
  • Be flexible.
    HawkZ05 Fast One-Step RT-PCR Kit enables amplification of both RNA and DNA targets.
  • Experience high performance.
    Achieve reliable amplification of your low-copy RNA targets due to high temperature reverse transcription at +60 to +65°C and improved RNA processivity.
  • Prepare stable amplification mixes in dry format.
    Use this formulation for producing dried-down amplification mixes stable at room temperature.
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Application
Apply HawkZ05 Fast One-Step RT-PCR Lyo Kit for:
  • Preparation of dried reaction mixes
  • High-throughput quantitative gene expression analysis
  • Target detection and quantification
  • Detection of rare transcripts
  • Reverse transcription and amplification of RNA from limited samples
  • Instruments requiring normalization with Rox
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Specification
Appearance: Clear, colorless solution
Function test: Average CT value of positive controls tested is between 20 and 30 cycles using starting template of 1x104 copy pAW 109 per reaction. Average CT value of real-time PCR test is within ±2 cycles of the proven specification.
Stability: At -15 to -25°C within specification range for 12 months.