Others Taq DNA Polymerase GMP Grade 5 U uL Taq DNA Polymerase, GMP Grade, 5 U/μl For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only acc. to Art. 56 (3) and 3 no. 23 REACH Regulation. 3.6.17.2.1.1 from Thermus aquaticus BM, expressed in E. coli, solution 03161455103 Taq DNA Polym GMP Grade 50ku Taq DNA Polymerase, GMP Grade, 5 U/uL Reagents, kits 50 kU 5 U/μL CustomBiotech product. Please contact your local representative. Will be supplied as "Taq DNA Polym GMP Grade 50ku". Unit of measure is "piece".The enzyme is supplied without reaction buffer. Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR. en Obtain consistent results. Rely on the robust reaction performance and the lot-to-lot consistency of this product. Stay ahead of regulatory requirements. High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations. en Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:Routine PCR and RT-PCR applicationsAmplification of DNA fragments up to 3 kb from various sources of DNALabeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactionsManufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control) en Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C. Volume activity: ≥5 U/μL Specific activity (Protein: A280): ≥130,000 U/mg Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. Purity (SDS PAGE): ≥98% Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Ribonucleases (MS2 RNA): Not detectable in up to 10 U after 1 hour incubation at +37°C. Function test in PCR (10 pg λDNA, 0.5 kb fragment): Corresponds to reference Function test in qPCR using LightCycler® (human genomic DNA, β-globin gene): Corresponds to reference (plasmid DNA, β-globin gene): Corresponds to reference Bioburden: ≤50 CFU/mL Animal-derived additives: None Stability: At -15 to -25°C within specification range for 24 months. en