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DNase I, recombinant, RNase-free

from bovine pancreas, expressed in Pichia pastoris, solution

For further processing only.

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Order information
DNase I, recombinant, RNase-free material number and pack size:
Material Number Pack Size
03539121103 custom fill
Will be supplied as "DNase I rec RNase free in Glycerol". Unit of measure is "kU".
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Overview
Recombinant DNase I is an essential tool for all applications requiring DNA-free RNA templates.
DNase I, recombinant, RNase-free, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a glycoprotein of a molecular weight of approximately 39 kD. DNase I, recombinant, RNase-free, is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
The enzyme is highly purified and rigorously tested for contaminating RNase and protease activity for superb RT-PCR.
  • Achieve reliable results with undegraded and stable RNA.
    Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high sensitivity of your transcription reaction.
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Application
Use DNase I, recombinant, for isolation of DNA-free RNA in diagnostic and therapeutic applications:
  • To ensure that RT-PCR templates are free of genomic DNA
  • To remove DNA templates after in vitro transcription of RNA
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Specification
Nomenclature: DNase I
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA.

Appearance: Colorless to slightly yellowish, solution
Volume activity (calf thymus DNA): 9-14 kU/mL
Volume activity (calf thymus DNA, modified buffer system): No limit
Proteases (up to 50 U resorufin-marked casein after 17 h; 37°C): Not detectable
Ribonucleases (up to 10 U with MS II RNA/4 h / 37 °C): Not detectable
Stability: At -15 to -25°C within specification range for 24 months.