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AMV Reverse Transcriptase, rec., concentrate

from Avian Myeloblastosis Virus, expressed in E. coli

For further processing only.

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AMV Reverse Transcriptase, rec., concentrate material number and pack size:
Material Number Pack Size
04460472103 200 kU
06676367103 20 kU
04460472103: Will be supplied as "AMV RT, rec., conc., 200 kU". Unit of measure is "kU".
06676367103: Will be supplied as "AMV RT, rec., conc., 20 kU". Unit of measure is "kU".
AMV Reverse Transcriptase with high thermostability for generation of RNA fragments up to 12 kb with high sensitivity.
Use AMV Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA for:
  • Two-step RT-PCR applications for amplification from RNA targets
  • RT-PCR for detection of viral RNA
  • TMA and NASBA nucleic acid amplification methods
  • Synthesis of full-length cDNA for libraries or cloning
  • Rapid amplification of cDNA end (RACE)
  • Manufacture of amplification mixtures for applications with regulatory requirements (e.g., in vitro diagnostics, quality control)
AMV Reverse Transcriptase provides higher thermostability compared to the native forms of AMV or M-MLV reverse transcriptase, allowing higher temperatures for reverse transcription, thus achieving better performance with GC-rich RNA fragments and difficult secondary structures.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, unwinding activity, RNase H (degrading RNA in RNA:DNA hybrids)
Recommended reaction temperature: +42 to +65°C
Substrates: Incorporates dNTP, ddNTP, dUPT, various labeled or modified nucleotides
Divalent ion requirement: Mg2+
Appearance: Clear, colorless solution
Storage buffer: Potassium phosphate, 200 mmol/L; DTT, 2 mmol/L; Triton X-100, 0.2% (v/v); glycerol, 50% (v/v); pH approximately 7.2
Volume activity: ≥80-150 U/μL
Specific activity: ≥50 kU/mg protein
Unit definition: One unit AMV Reverse Transcriptase, recombinant, is defined as the amount of enzyme which incorporates 1 nmol of [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.
Purity (SDS PAGE): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 25 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 40 U after 4 hours incubation at +37°C.
Function test in RT-PCR (human skeletal muscle total RNA, 10 kb dystrophin gene fragment): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 12 months.