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Alkaline Phosphatase, recombinant, 20 U/μL

from bovine intestine, expressed in Pichia pastoris, solution

For further processing only.

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Order information
Alkaline Phosphatase, recombinant, 20 U/μL material number and pack size:
Material Number Pack Size
04571363103 custom fill 20 U/μL
Will be supplied as "Alkaline Phosphatase rec., MB Grade, 20 U". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
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Overview
Alkaline Phosphatase for 5' dephosphorylation of DNA and RNA.
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Application
Use Alkalkine Phosphatase, recombinant for:
  • Dephosphorylation of 5' phosphate from DNA and RNA
  • Coupling to other proteins via its amino or carbohydrate groups, for detection in immunoassays and western blot analysis
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Specification
Enzyme activity: Alkaline Phosphatase, recombinant, 1 U/μL catalyzes the dephosphorylation of 5' phosphate from DNA and RNA.
pH activity optimum: 9.8
pH stability optimum: 8.0
Cofactor: Zn2+
Aktivators: Mg2+, Mn2+, Co2+
Inactivation: Complete inactivation after 5 minutes at +75°C.
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; MgCl2, 1 mmol/L; ZnCl2, 0.1 mmol/L; glycerol, 50% (v/v); pH approximately 7.6 at +4°C
Volume activity: ≥20 U/μL
Specific activity: ≥5 kU/mg protein
Unspecific endonucleases (λDNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 60 U after 4 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 100 U after 1 hour incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.