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Biotin-16-UTP

10 mM solution

For further processing only.

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Order information
Biotin-16-UTP material number and pack size:
Material Number Pack Size
11413201103 custom fill
Will be supplied as ''Biotin-16-UTP''. Unit of measure is ''nmol''.
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Overview
Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates.
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Application
Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA Polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and Biotin-16-UTP, respectively.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate.
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Specification
Nomenclature: Biotin-16-uridine-5'-triphosphate
Formula: C32H48N7O19P3SLi4
Molecular weight: 987.5 D
Appearance: Clear, colorless solution
Biotin-16-UTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 10.0-11.0 mmol/L
Purity (HPLC): ≥85 area%
Function test using DIG RNA Labeling Kit (SP6/T7) (Sensitivity Dot Blot ≤0.1 pg): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.