1 mM solution
For further processing only.
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Digoxigenin-11-ddUTP material number and pack size: Material Number Pack Size 11365207103 custom fill
For other than the listed concentrations please inquire.
- Digoxigenin-11-ddUTP permits replacement of radioactive assays for even higher sensitivity. The required exposure time is drastically reduced compared to a radioactive assay.
- Use Digoxigenin-11-ddUTP as a substrate for: Terminal Transferase, DNA polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerase or Taq DNA polymerase and reverse transcriptase (e.g., Transcriptor). It is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant. The DIG-labeled oligonucleotide can be used as a hybridization probe for:
- DNA and RNA transfers
- Colony and plaque screening
- In situ hybridization
Molecular weight: 1074.7 D Appearance: Clear, colorless solution
Digoxigenin-11-ddUTP (1 μmol ≙ 8.78 A290 units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L
Purity (HPLC): ≥85.0 area%
Function test of completeness of labelings in Agilent Bioanalyzer by bands shift in reference to the unlabeled oligonucleotide: 90-100%
Function test using DIG Oligonucleotide 3'-End Labeling Kit, 2nd generation: Corresponds to reference
Stability: At -15 to -25°C within specification range for 24 months.