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Digoxigenin-11-ddUTP

1 mM solution

For further processing only.

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Order information
Digoxigenin-11-ddUTP material number and pack size:
Material Number Pack Size
11365207103 custom fill
Will be supplied as ''DIG-11-ddUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire.
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Overview
Digoxigenin-11-ddUTP permits replacement of radioactive assays for even higher sensitivity. The required exposure time is drastically reduced compared to a radioactive assay.
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Application
Use Digoxigenin-11-ddUTP as a substrate for: Terminal Transferase, DNA polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerase or Taq DNA polymerase and reverse transcriptase (e.g., Transcriptor). It is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant. The DIG-labeled oligonucleotide can be used as a hybridization probe for:
  • DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, DIG labeled oligonucleotides are specifically useful for screening expression libraries for sequence specific DNA-binding proteins such as transcription factors.
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Specification
Nomenclature: Digoxigenin-11-2'-dideoxy-uridine-5'-triphosphate
Formula: C43H61N4O20P3Li4
Molecular weight: 1074.7 D
Appearance: Clear, colorless solution
Digoxigenin-11-ddUTP (1 μmol ≙ 8.78 A290 units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L
Purity (HPLC): ≥85.0 area%
Function test of completeness of labelings in Agilent Bioanalyzer by bands shift in reference to the unlabeled oligonucleotide: 90-100%
Function test using DIG Oligonucleotide 3'-End Labeling Kit, 2nd generation: Corresponds to reference
Stability: At -15 to -25°C within specification range for 24 months.